Figure 3.
PTB-AS Could Significantly Promote the Proliferation and Migration of Glioma both In Vitro and In Vivo
(A and B) MTS and colony formation assays showing that PTB-AS, similar to PTBP1, promotes cell viability. (A) The growth curve of T98G cells after knocking down or overexpressing PTB-AS or PTBP1, following individual tests of the absorbance at 490 and 630 nm. (B) Colony formation images and bar charts of the number of colonies. Data are expressed as the mean ± SD, n = 5. *p < 0.05, **p < 0.01 (Student’s t test). (C and D) Wound-healing assays and Transwell migration assays showing that PTB-AS and PTBP1 both promote the migration of glioma cells. (C) The relative area of the remaining open wound calculated in relation to that at time 0 h. (D) The graphs indicate the average number of cells per field of the indicated cell lines in migration assays. Data are the mean ± SD. *p < 0.05, **p < 0.01 (Student’s t test). (E) In vivo assays were performed using shNC and shPTB-AS stable lentivirus infection U87MG cell suspensions (5 × 105 cells/5 μL). The cells were injected intracranially into five nude mice. H&E staining was processed after perfusion and paraffin preparation. The circles in the violet areas indicate the tumor. (F) An immunoblot analysis was performed to show the expression of mesenchymal markers, epithelial markers, and EMT-related transcription factors after knocking down PTB-AS, and representative immunohistochemistry was performed to detect Vimentin in shNC- and shPTB-AS-infected cells in vivo. Scale bars: 200 and 50 μm.