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. 2019 Jun 14;27(9):1653–1664. doi: 10.1016/j.ymthe.2019.06.005

Figure 3.

Figure 3

miR-1251-5p Drives Cell Cycle Progression

(A and B) Cell cycle distributions of HEY and SKOV3 cells transfected with NC or miR-1251-5p inhibitor. Representative images (A) and quantitative data (B) are shown. Green, yellow, and blue represent the populations of cells in G0/G1, S, and G2/M phases, respectively. (C and D) Treated cells were cultured for 48 h, harvested, fixed using ethanol, and stained with PI. Cell cycle distributions of HEY and SKOV3 cells stably transfected with empty vector or miR-1251-5p expression vector were assessed using flow cytometry. Representative images (C) and quantitative data (D) are shown. Green, yellow, and blue represent the populations of cells in G0/G1, S, and G2/M phases, respectively. *p < 0.05, **p < 0.01, ***p < 0.001.