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. 2019 Jun 14;27(9):1653–1664. doi: 10.1016/j.ymthe.2019.06.005

Figure 5.

Figure 5

Knockdown of TBCC Promotes Cell Proliferation and Cell Cycle Progression and Induces Autophagy

(A) Proliferation of cells with or without TBCC silencing. (B) Colony formation of cells with or without TBCC silencing. Representative images and quantitative data are shown. (C and D) Cell cycle distributions of cells with or without TBCC silencing. Representative images (C) and quantitative data (D) are shown. Green, yellow, and blue represent the populations of cells in G0/G1, S, and G2/M phases, respectively. (E) Protein levels of TBCC, LC3B, and p62 in TBCC-silencing or control cells. Representative images and quantitative data are shown. (F) Representative images of cells with or without TBCC silencing by immunofluorscent staining of DNA (DAPI, blue), TBCC (red), and LC3B (green). Autophagosome formation was monitored via confocal microscopy. (G) Autophagosome formation was monitored via confocal microscopy in TBCC-downexpressing cells treated with 25 μM chloroquine for 8 and 10 h, respectively. *p < 0.05, **p < 0.01, ***p < 0.001.