Figure 2.

Primary Tracheal Cultures of Rice Rats Have Limited Basal and Amiloride-Sensitive Current, but Large Carbachol-Mediated Cl⁻ Secretion

(A–H) Basal short circuit current (Isc) measurements in primary cultures of rice rat and piglet (A). Averaged delta (Δ) Isc measurements shown for 100 μM apical amiloride (AMIL) (B), 100 μM basolateral carbachol (CCh) (peak and sustained responses in C and D, respectively), 30 μM apical CaCCinhi-A01for inhibition of peak (E) and sustained (F) CCh-mediated current, apical 10 μM forskolin and 100 μM IBMX (F&I) (G), and 100 μM apical GLYH-101 (H).

(I–K) Representative short circuit current (Isc) trace in mouse (I), piglet (J), and rice rat (K) airway cultures. Arrows indicate addition of drugs (as described in Methods). CCh, carbachol; F&I, forskolin and IBMX. For all panels, n = 4 cultures from mice (2 female cultures and 2 male cultures representing 6 female and 6 male subjects); n = 7 cultures from the rice rat (4 female cultures and 3 male cultures representing 8 female and 6 male subjects); n = 9 porcine cultures (5 female and 4 male representing 5 individual female and 4 individual male subjects). ∗p < 0.05 compared with mouse; ^#p < 0.05 compared with pig. All data are shown as mean ± SEM.