Fig. 1.

Mouse pedigree sequencing and genotyping strategy. Six reciprocal crosses were established and successively mated, generating six pedigrees comprising between 41 and 77 offspring. The pedigrees shown here only include the sequenced and genotyped individuals. Three tissues (spleen, kidney and tail) were collected from all mice. Whole-genome sequencing (WGS) was performed on spleen-derived DNA for five or ten pups within each pedigree (shown in red). Candidate DNMs were identified and validated using deeper targeted sequencing of 1–3 tissues per individual across the offspring shown. The naming conventions and differences between data collection and analysis are shown. Not all offspring from each pedigree were genotyped