Fig. 3.

Stability and reusability of RIMRs. a Storage stability (incubation at 4 °C) of immobilized (red line) and free RuBisCO (dark line). b Thermal stability of immobilized (red line) and free RuBisCO (dark line). All samples are incubated for 10 min before the activity assay. c Reusability of the RIMRs when RuBP is injected at 1.4 μL min⁻¹ (the reaction time is 5 min). Red dash-dotted line is the third-order polynomial fitting, representing that the activity drop trend tends to slow down with the increase of cycles of reuse. d Relative activity as a function of the flow rate of RuBP injection (from 7 to 0.7 μL min⁻¹) for repeated uses. The amount of RuBisCO is 21.875 μg for both the immobilized and free ones in all the experiments. The volume of the collected production solutions for the storage and thermal stability tests are 100 μL. RuBP concentration is 0.5 mM and ${\mathrm{HCO}}_3^{-}$ in the reaction buffer is 66 mM. Error bars represent the standard deviations from three independent experiments. Source data are provided as a Source Data file