Fig. 4.

Continuous production of 3-PGA as a function of the volume of collected production solution. The dark open squares represent the 3-PGA production in the bulk reaction with free RuBisCO and the red solid circles represent the 3-PGA production in the RIMRs with the immobilized RuBisCO. The amount of RuBisCO used is 21.875 μg for both the immobilized and free ones. RuBP concentration is 0.5 mM and ${\mathrm{HCO}}_3^{-}$ in the reaction buffer is 66 mM. Reaction temperature is 30 °C. Reaction time is 1 min. Error bars represent the standard deviations from three independent experiments. Source data are provided as a Source Data file