Table 1.

Kinetic parameters obtained from RIMRs reaction and bulk reaction^a

Reaction type	Vmax (mmol min−1 g−1 RuBisCO)	Km (RuBP) (mM)
RIMRs reaction	0.070 ± 0.003	0.070 ± 0.012
Bulk reaction	0.169 ± 0.006	0.049 ± 0.008

^aThe collected production solutions I and II are both 100 μL. RuBP concentrations are 0.01–2 mM for the bulk reaction and 0.025–2 mM for the RIMRs reaction. The concentration of bicarbonate $\left({\mathrm{HCO}}_3^{-}\right)$ in the reaction buffer is 66 mM. K_m and V_max values are the means ± s.d. of three independent experiments, which is calculated by the GraphPad Prism 7 according to the nonlinear fitting of Michaelis–Menten model. Source data are provided as a Source Data file