Table 2.
Reaction type | Storage stabilitya | Thermal stabilityb | Reusabilityc | Production of 3-PGA (μmol g−1 RuBisCO) |
---|---|---|---|---|
RIMRs reaction | 43% | 67% | 90.4% | |
Bulk reaction | 6% | 10% | – | Saturation at ~360 |
Enhancement factor | 7.2-fold | 6.7-fold | – | 1.8-folde |
aStorage stability is defined as the remaining relative activity after 15 days
bThermal stability is the remaining relative activity after incubation at 70 °C for 10 min
cReusability is the remaining relative activity after five cycles of reuse at the flow rate of 1.4 μL min−1
dVp is the volume of the reactant mixture (RuBP and in the reaction buffer)
eData is obtained when Vp is 1680 μL