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. 2019 Sep 6;10:4065. doi: 10.1038/s41467-019-11942-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — ESRRB O-GlcNAcylation enhances self-renewal and pluripotency. a Clonogenicity assay of mESCs stably expressing Mock, FLAG-ESRRB^WT and FLAG-ESRRB^S25A. Bar graphs showing the statistical analysis of the undifferentiated, mixed and differentiated colonies in the absence of LIF for 6 days (left panel) and 9 days (right panel). Error bars represent mean ± s.d. Results are from three independent experiments. **P < 0.01, ***P < 0.001, n.s., not significant (one-way ANOVA). b Teratoma formation in immunodeficient mice by mESCs expressing FLAG-ESRRB or FLAG-ESRRB^S25A. c Box plot showing the statistical analysis of teratoma weight. Median and quartile values are provided by the central line and box boundaries. Whiskers show min to max values. Results are from ten independent experiments. **P < 0.01 (Student’s t-test). Source data for figures a, c are provided as a Source Data file