Fig. 1.

Unanticipated gene expression outcomes following on-target CRISPR editing. a The effect of CRISPR-introduced frameshift alterations on mRNA and protein expression was analyzed using a panel of CRISPR-Cas9-edited HAP1 cells that were commercially accessible. The targeted exon, anticipated PTC location following insertion/deletion mutation and the protein recognition sites of antibodies used in panel b are indicated. b Appearance of novel proteins in cells edited with CRISPR-Cas9. HAP1 cells were subjected to western blot analysis using two distinct antibodies. Asterisks (*) indicate novel proteins. c CRISPR-Cas9 gene editing induces expression of novel mRNA species. RT-PCR analysis of edited cells was performed using primers recognizing flanking exons and the amplicons generated were sequenced. Asterisks (*) indicate novel mRNA species. Source data are provided as a Source Data file