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. 2019 Sep 6;9:12827. doi: 10.1038/s41598-019-49289-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phlda3 mRNA and protein levels are upregulated by thapsigargin treatment in parallel with the induction of ER stress genes. MIN6 cells were cultured for 24 h in the absence (white bars) or presence of 300 nmol/l (grey bars) or 1000 nmol/l (black bars) thapsigargin (Tg). Changes in the mRNA levels of Phlda3, Hmox1, Trb3, Hspa5, Hsp90b1 and Fkbp11 (a–f) and PHLDA3 immunostaining (g). *p < 0.05, ***p < 0.001 vs control. ^###p < 0.001 vs thapsigargin 300 nM. Scale bar, 50 µm.