Fig. 5. miR-214-3p suppresses oncogenic pathway by targeting fibroblast growth factor receptor 1 (FGFR1).

a, c A gene set enrichment analysis related to the epithelial–mesenchymal transition (EMT) signatures and hallmarks in the groups with high and low expression of miR-214-3p or FGFR1 in the The Cancer Genome Atlas (TCGA) lung cancer cohort. The signaling pathways were negatively regulated by miR-214-3p and positively regulated by FGFR1. Wnt signaling pathway as an intersection was determined by a normalized enrichment score (NES). b The mRNA level of β-catenin, c-Myc, and cyclinD1 in Wnt signaling in the groups with high and low expression of miR-214-3p or FGFR1 in the TCGA lung cancer cohort. d After transfection of miR-NC and miR-214-3p mimic or anti-miR-NC and anti-miR-214-3p for 24 h, the protein levels of FGFR1, pFGFR1, MAPK (mitogen-activated protein kinase), phosphoinositide 3-kinase-AKT (PI3K-AKT), and Wnt signaling pathway were measured by western blot, and the expression level of FGFR1 were measured by western blot after transfection for 48 h. e After transfection of FGFR1 overexpression plasmid or small interfering RNA (siRNA) of FGFR1 for 48 h, the protein levels of Wnt signaling pathways were measured by western blot. f After transfection of miR-214-3p or FGFR1 plasmid or co-transfection of miR-214-3p and FGFR1 plasmid for 48 h, the protein levels of FGFR1, pFGFR1, MAPK, AKT, and Wnt signaling pathways were measured by western blot. P values were calculated by Student’s t test: *p < 0.05; **p < 0.01