Fig. 5.

Mis18^core forms stable complex with only one copy of HJURP. a–f Analytical SEC experiments were performed to confirm that HJURP fragments bind full-length Mis18^core or Mis18α^56–C:Mis18β without dissociation of these complexes. SDS-PAGE gels were stained with CBB. g Sedimentation coefficient distributions obtained from the sedimentation velocity AUC experiments using the purified samples described in Supplementary Fig. 6a, b. Data profiles used for curve-fitting analyses are shown in Supplementary Fig. 6c. h A table showing the values obtained from the AUC experiments in panel g. Sed. coef. sedimentation coefficient, MW_obs. observed molecular weight, MW_theo. theoretical molecular weight