Figure 4.

Putative Susceptibility Genes Associated with TMB of Cancer-Driver Genes

(A) Mutation spectrum of cancer-driver genes with high alteration frequency (≥6%) in each sample across cancer types. The top boxes with different colors indicate samples of different cancer types. The lines in different colors indicate the mutation of each driver gene in each sample. The carcinogenesis signaling pathways for these driver genes are indicated with different colors on the left.

(B) Scatterplots for TMB of cancer-driver genes for each sample across seven cancer types. Each dot represents each sample and “n” refers to sample size. The red line indicates the median of TMB of cancer-driver genes for each cancer type.

(C) Bar plots showing the statistical significance between expression levels of putative susceptibility genes and TMB of cancer-driver genes across seven cancer types. The dashed lines indicate the genes with statistical associations at Bonferroni-corrected p < 0.05 in each cancer type.

(D) Violin plots of samples separated by low, median, and high expression levels of the highlighted genes (see Results); the genes were associated with TMB of cancer-driver genes at Bonferroni-correction p < 0.05. The upper plots show the associations in breast cancer, the lower plots show the associations in colorectal cancer.

(E) Heatmap plots showing the putative susceptibility genes associated with both TMB of cancer-driver genes and mutational signatures in breast and colorectal cancer. The ↑ and ↓ refer to a positive and negative association, respectively. The lower plots show the correlation coefficients between TMB of cancer-driver genes and mutational signatures in breast and colorectal cancer, respectively.