Figure 2.

Determination of the photobleaching rate and binding lifetime from subsampling experimental data. (A) Representative CRTDs when only 3, 10, or 30% of experimental trajectories were randomly selected. Counts can be approximated as y intercepts of exponential fits of CRTDs. The CRTD from the full data set (right-most panel) is reproduced from (32). (B) Shown are k_effτ_tl plots of the corresponding CRTDs (above). Shaded error bands are SDs from 10 bootstrapped samples. (C) Scatter plots show distributions of k_b obtained using the global fitting of 100 subsets of the experimental data at the indicated fraction. Each point represents the average of results from 10 bootstrapped samples. (D) Scatter plots show distributions of τ obtained using the global fitting of 100 subsets of the experimental data at the indicated fraction. Similarly, each point represents the average of results from 10 bootstrapped samples. Bars and boxes represent means and SDs of the fitting outcomes of 100 subsets of the data set, respectively. The experimentally measured value of τ = 17.9 ± 0.9 s for the entire data set is reproduced from (32). To see this figure in color, go online.