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. 2019 Jul 19;117(5):950–961. doi: 10.1016/j.bpj.2019.07.015

Figure 4.

Figure 4

Determination of binding lifetimes and amplitudes from biexponential distributions with an intermediate rate (koff1) and a fast rate (koff2 = 10 koff1). (A–D) Shown are keffτtl plots of biexponential distributions with kbτint of 0.7, koff1 and koff2 of 0.1 and 1.0 s−1, respectively, with (A) 1 × 103, (B) 3 × 103, (C) 1 × 104, or (D) 1 × 105 observations. The amplitude of koff1 (B) is 10%, 25%, 50%, or 90%. Shaded error bars are SDs from 10 bootstrapped samples. (E–G) Heatmaps show errors in estimates of B, τ1, and τ2 obtained using the global fitting of 100 simulated distributions for each n value (see Fig. S4 for distributions). To see this figure in color, go online.