Figure 5.

Determination of binding lifetimes and amplitudes from biexponential distributions with a slow rate (k_off1) and an intermediate rate (k_off2 = 10k_off1). (A–C) Shown are k_effτ_tl plots of biexponential distributions with k_bτ_int of 0.7, k_off1 and k_off2 of 0.01 and 0.1 s⁻¹, respectively, with (A) 1 × 10³, (B) 1 × 10⁴, or (C) 1 × 10⁵ observations. The amplitude of k_off1 (B) is 10%, 25%, 50%, or 90%. Shaded error bars are SDs from 10 bootstrapped samples. (D) Scatter plots show a distribution of apparent τ (τ^∗) obtained from the fitting of 100 simulated biexponential distributions at a specified B and 1 × 10⁵ counts to a monoexponential model. Line is the exponential fit between the average of τ^∗ (represented by bars) and B. (E–G) Heatmaps show errors in estimates of B, τ₁, and τ₂ obtained using the global fitting of 100 simulated distributions for each n value (see Fig. S6 for distributions). To see this figure in color, go online.