Figure 7.

Determination of binding lifetimes and amplitudes from triexponential distributions with a slow rate (k_off1), an intermediate rate (k_off2 = 10k_off1), and a fast rate (k_off3 = 10k_off2), using the 100-s τ_tl set. From left to right, five panels in each row correspond to different amplitudes of each subpopulation (displayed on top). (A) Shown are k_effτ_tl plots of triexponential distributions with k_bτ_int of 0.7 and k_off1, k_off2, and k_off3 of 0.01, 0.1, and 1 s⁻¹, respectively, with 1 × 10⁶ observations. Shaded error bands are SDs from 10 bootstrapped samples. (B–F) Heatmaps show errors in estimates of B₁, B₂, τ₁, τ₂, and τ₃ obtained using the global fitting of 100 simulated distributions at various preset values of B₁ and B₂ (see Fig. S9 for distributions). To see this figure in color, go online.