Figure 9.

Dynamic range and resolution limits in resolving multiple populations using the time-lapse imaging technique with photobleaching-prone fluorescent probes. Dynamic range limit is a function of the photobleaching rate (k_b) and the maximal τ_tl (τ_{tl_max}) used in experimental conditions. τ_n−1 is the longest binding lifetime in a multiexponential distribution within pairs of off rates k_n and k_n−1. Shaded zones indicate conditions in which errors in estimates of τ and the amplitude are high. To see this figure in color, go online.