Figure 3.

miR-876-3p overexpression blocks invasion and migration of glioma. A and C. Migration of glioma cells was monitored by a wound-healing assay. Representative images of glioma cells transfected with miR-NC or miR-876-3p. Data were expressed as the mean ± SEM from three independent experiments. *P < 0.05. B and D. Migration of glioma cells was monitored by a Matrigel invasion assay. Representative images of glioma cells transfected with miR-NC or miR-876-3p. Data were expressed as the mean ± SEM from three independent experiments. *P < 0.05. E. Results of glioma cell invasion were validated by a 3D spheroid migration assay. F. EMT-associated proteins in miR-NC or miR-876-3p transfected U251 or T98 cells were determined by western blotting. Actin was used as the loading control.