KIF20A reintroduction reverses the inhibitory effect of miR-876-3p. A. Western blot analysis of KIF20A, p-JAK2, and p-STAT3 protein levels in cells transfected with KIF20A in the presence of miR-NC or miR-876-3p. B. Effect of KIF20A reintroduction on miR-NC or miR-628-5p transfected U251 and T98 growth rates as measured by CCK-8 assay. Data were expressed as the mean ± SEM from three independent experiments. *P < 0.05, #P < 0.05. C, D and F. Effect of KIF20A reintroduction on miR-NC or miR-876-3p transfected on U251 and T98 migration ability as determined by wound-healing assay. Data were expressed as the mean ± SEM from three independent experiments. *P < 0.05, #P < 0.05. E and G. Effect of KIF20A reintroduction on miR-NC or miR-876-3p transfected on U251 and T98 invasion ability as determined by a Matrigel invasion assay. Data were expressed as the mean ± SEM from three independent experiments. *P < 0.05, #P < 0.05.