Figure 4.

DHX9 knockdown promoted the proliferation, migration, and invasion of lung adenocarcinoma. A. Cell migration monitored by “wound” healing assay at zero, 24, and 48 hours. Pictures showed that SH1 cells migrated more to the scratches at 24 and 48 hours than the control and negative control. B. Wound area was calculated using Image-Pro Plus. SH1 cells covered 73% of the gap at 24 hours and 85% at 48 hours, and NC cells covered 18% at 24 hours and 25% at 48 hours. C. Cells were seeded in 96-well plates. After incubation for 24, 48, and 72 hours, cells were treated with MTT for another four hours. The absorbance at 490 nm was measured. **P < 0.01, when compared with the control group. D, E. Transwell migration and invasion assays on the indicated cells. The migration assay showed that the migrated cells were 475.40 ± 57.05 for SH1 and 144.10 ± 5.77 for NC. The invasion assay showed that the invaded cells were 428.00 ± 11.07 for SH1 and 142.30 ± 11.74 for NC. DHX9 knockdown promoted cell migration and invasion of LAC. **P < 0.01, ***P < 0.001.