Figure 8.
Functional changes in GMCs after interference with mmu_circ_0001605 and mmu_circ_0000372. A: QRT-PCR confirmation of the results of knockdown treatment with mmu_circ_0001605 and mmu_circ_0000372 in GMCs. B: ELISA examination of the expression of Col IV in GMCs. C: Cell proliferation analysis of GMCs after knockdown by mmu_circ_0001605 and mmu_circ_0000372. D: Immunofluorescence analysis of α-SMA in GMCs after knockdown by mmu_circ_0001605 and mmu_circ_0000372; Scale bar = 50 µm circRNF169 refers to mmu_circ_0001605, located between chr7: 107083672-107104050. circSTRN3 refers to mmu_circ_0000372, located between chr12:52748981-52762700. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. siRNA-NC: GMC treated with nonsense siRNA; si-circRNF-169: mmu_circ_0001605 siRNA-treated GMCs; si-circSTRN3: mmu_circ_0000372 siRNA-treated GMCs.