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. 2019 Aug 15;11(8):5065–5075.

Figure 2.

Figure 2

EndoA2 attenuated hypertrophic responses induced by ISO in neonatal rat cardiomyocytes (NRCMs). A, B. NRCMs were transfected with Ad-EndoA2 or siRNA followed by incubation with ISO (1 μM for 24 h). Morphological changes were observed by staining with α-actinin. ISO increased cell surface area, whereas EndoA2 overexpression decreased the ISO-induced cell surface area, and EndoA2 siRNA knockdown further enhanced the effects of ISO on the cell surface area. n=6, *P<0.05 vs. control group, #P<0.05 vs. ISO group. C. The mRNA expression levels were determined by qPCR. Statistical analyses showed that ISO increased the mRNA expression levels of ANF, BNP and β-MHC, whereas EndoA2 overexpression significantly decreased the ISO-induced increases in ANF, BNP and β-MHC mRNA expression levels in NRCMs, EndoA2 siRNA knockdown further enhanced the effects of ISO on the mRNA expression levels of ANF, BNP and β-MHC. n=6, *P<0.05 vs. control group, #P<0.05 vs. ISO group.