Figure 4.

EndoA2 overexpression-induced autophagy accounted for the protective effects of EndoA2 on cardiac hypertrophy induced by ISO. A-C. NRCMs were treated with the autophagy inhibitor 3-MA (1 mM, 24 h). Immunofluorescence and qPCR were employed to determine the hypertrophic response in NRCMs. Representative images of cell surface area and the densitometric analyses of qPCR showed that pretreatment with 3-MA abrogated the protective effects of EndoA2 on ISO-induced cardiac hypertrophy, as evidenced by increased cell surface area and mRNA expression levels of ANF and BNP compared with the ISO+Ad-EndoA2 group. n=6, *P<0.05 vs. control group, #P<0.05 vs. ISO group, **P<0.05 vs. ISO+Ad-EndoA2 group.