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. 2019 Aug 15;11(8):4976–4983.

Figure 5.

Figure 5

Wnt2 is a direct target of miR-30a-3p. A. TargetScan identified a highly conserved putative miR-30a-3p binding site in the 3’-UTR of Wnt2. The corresponding sequences of a miR-30a-3p mimic and a NC mutant were designed and synthesized. B. In luciferase reporter assays, overexpression of miR-30a-3p reduced luciferase activity in the presence of a WT Wnt2 reporter gene, but not in the presence of a mutated reporter gene, in 786-O cells. C. Overexpression of miR-30a-3p inhibited transcription of Wnt2 mRNA in 786-O cells. D. Western blot analysis of Wnt2 and β-catenin expression in 786-O cells transfected with miR-30a-3p and NC. Detection of actin served as a loading control. The data presented are the mean ± SD. *P < 0.05. NC, negative control.