Figure 6.

Ectopic overexpression of Wnt2 reverses the anti-tumor effects induced by miR-30a-3p in 786-O cells. A. Western blot analysis of Wnt2 in 786-O cells transfected with the indicated constructs. Actin was detected as a loading control. B. MTT proliferation assay results for 786-O cells transfected with the indicated constructs. C. Number of 786-O colonies formed after transfections with the indicated constructs. D. Cell cycle distribution of 786-O cells after transfections with the indicated constructs. E. Wound healing assay results for 786-O cells after transfections with the indicated constructs. F. Cell migration data from Transwell chamber assays after transfection of 786-O cells with the indicated constructs. All data are presented as the mean ± SD. *P < 0.05. NC, negative control.