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. 2019 Aug 15;11(8):5096–5104.

Figure 4.

Figure 4

Inhibitory effect of vitisin B on FAS expression and intracellular FAS activity in MDA-MB-231 cells. A. Effect of vitisin B on FAS mRNA. MDA-MB-231 cells were treated with 0, 2, 4, 8 μg/ml vitisin B. After 24 h, mRNA was extracted and quantified via RT-PCR, and normalized to β-actin mRNA. Data were normalized to control cells without vitisin B (0 μg/ml). Data was analyzed using unpaired Student’s t test, **P < 0.01 compared to the control (0 μg/ml). B. Representative pictures for FAS protein expression by western blot analysis. Cells were treated with 0, 2, 4, 8 μg/ml vitisin B. Vitisin B down-regulated FAS expression in MDA-MB-231 cells in a dose-dependent manner. Shown gels were representative of those obtained from at least three independent experiments. C. Intracellular FAS activity measured in MDA-MB-231 cells using NADPH by spectrophotometry at 340 nm. Relative FAS activities were represented as the means ± SD from three independent experiments with similar results. *P < 0.05 compared to the control (0 μg/ml).