Figure 3.

HSF1 was involved in the conversation of energy metabolism between TAMs and HCC cells. When MHCC97H cells were cocultured with TAMs, the expressions of MCT1 and MCT4 were separately detected and relatively quantitative analysis was performed (A, B). The protein levels of MCT1 in HCC cells were analyzed by Western blotting after adding L-lactate (5 mM) in glucose-restriction (1 g/L) condition at indicated times (C). After TAMs were treated with shRNA against MCT4 (shMCT4-TAMs), expressions of HSF1, MCT1 and MCT4 in TAMs were analyzed by Western blotting (D, E) and proliferation of TAMs was determined by CCK-8 assay (F). In addition, lactate release rate of TAMs was determined after transfection (G). When MHCC97H cells were co-cultured with shMCT4-TAMs, ROS level was detected by relevant Kit according to the manufacturer’s instructions (H) and MCT1, MCT4 and HSF1 expression in MHCC97H cells was detected by Western blotting (I, J). *P<0.05 or **P<0.01 vs Mock.