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. 2019 Aug 15;11(8):5249–5260.

Figure 2.

Figure 2

SPAG9 depletion inhibits prostate cancer cell migration and its over-expression promotes cell migration. A. The protein expression of SPAG9 in three prostate cell lines PC-3, DU145, C4-2; B. Western blot analysis showed that SPAG9-targeting sh-RNAs in DU145 cell line and SPAG9 over-expression plasmid in PC-3 cell line to confirm knockdown and transfection efficiency; C. Effects of SPAG9 over-expression on PC-3 cell migration in vitro. **P<0.001 compared with control; The affection of SPAG9 Sh-RNA on DU145 cell migration in vitro. ***P<0.0001 compared with Negative control Sh-RNA. D. PC-3 cell transfected with SPAG9 over-expression showed significantly increased cellular motility even compared with the control group after 48 h. Observations based on three experimental triplicates.