Skip to main content
. 2019 Aug 15;11(8):5249–5260.

Figure 3.

Figure 3

SPAG9 promotes the prostate cancer cells proliferation. A. Prostate cancer cells were treated as indicated, cells were seeded in 96-well plates for CCK-8 assays or seeded in 6-well plates for fluid cytology technology tests. CCK-8 assay in PC-3 and DU145 cells transfected with SPAG9 over-expression or SPAG9-ShRNA at the indicated time points; *P<0.05. B. SPAG9 over-expression was shown of Flow cytometric in PC-3 cell lines, Cell cycle were accelerated at S phase in SPAG9 over-expression transfected cells as compared with control transfected cells. SPAG9-ShRNA was shown of Flow cytometric in DU145 cell lines, Cell cycle were blocked at S phase compared with negative control transfected cells. *P<0.05. C. CyclinD1 and CDK2 expression were regulated by SPAG9 in PC-3 and DU145 cell lines.