Figure 5.
Silencing of SPAG9 inhibits tumor cell growth in vitro. DU145 cells (1×106) stably expressing SPAG9 shRNA and Control shRNA were injected subcutaneously into the right backside of the nude mice. A. The representative photo of showing nude mice with tumor (arrows) treated with control shRNA or SPAG9 shRNA. B, C. The average volume and weight of was shown in the SPAG9-silenced DU145 compared with the control group (n=3, *P<0.05). D. H&E staining of the mice tumor and immunohistochemical staining expression of SPAG9 and ki-67 (*P<0.05, **P<0.01, ***P<0.001).