FIGURE 3.

LPS stimulation induces c-Abl’s nuclear transportation. (A) LPS stimulation induces nuclear import of c-Abl. RAW 264.7 cells were challenged with LPS for various lengths of time. Cell lysates from nuclear and cytoplasmic fractions were subjected to Western blotting to determine the subcellular distribution of c-Abl. (B) Immune-fluorescence staining verifies LPS-induced nuclear import of c-Abl. RAW 264.7 cells were mock treated or LPS exposed (±STI571) for 1 h, and then cells were fixed and permeabilized and incubated with anti–c-Abl rabbit polyclonal Ab and TRITC-conjugated secondary Ab. The nuclei of the cells were stained with DAPI. Similar results were obtained from at least three independent experiments. Original magnification ×180.