FIGURE 6.

c-Abl activity promotes RelA/p65 binding with PARP1 and PARyaltion (A and B) c-Abl activity promotes RelA/p65 binding with PARP1. RAW 264.7 cells were mock treated or exposed to LPS (±STI571) for 1 h. Immunoprecipitation was performed, and Ab against PARP1 (A) or RelA/p65 (B) was used to get precipitate complexes. Western blotting was performed using Abs specific for p65 or PARP1 in the complexes, respectively. (C and D) c-Abl activity promotes RelA/p65 PARyaltion. (C) RAW 264.7 cells were treated as described above. Immunoprecipitates were obtained by using Ab against RelA/p65; PARylation levels of RelA/p65 were determined by Western blotting using Ab recognizing PAR. (D) RAW 264.7 cells were transfected with siRNA targeting c-Abl or the control for 48 h and then challenged with LPS for 1 h. Immunoprecipitates were obtained using Ab recognizing RelA/p65. PARylation levels of RelA/p65 in the presence or absence of c-Abl were detected by Western blotting. Similar results were obtained from at least three independent experiments.