FIGURE 9.

Proinflammatory genes’ mRNA expression is impaired in Y829F PARP1-expressing cells. Endogenous PARP1 in Raw 264.7 cells was silenced using siRNA targeting a distinguished sequence of PARP1, and human WT PARP1, Y829F PARP1, and Y907F PARP1 expressional plasmids were transfected and then the cells were stimulated with LPS or not for 1 h. Immunoblotting was performed to detect the interference of endogenous PARP1 as well as the ectopic expression of Flag-tagged WT, Y829F, and Y907F PARP1 (A). Raw 264.7 cells as described above were used; proinflammatory genes’ mRNA levels were detected by RT-PCR and electrophoresis (upper) or real-time PCR (lower) (B). Data were expressed as mean ± SD. Difference significance was analyzed by one-way ANOVA. **p < 0.01.