Figure 6.

Direct detection of two caspases in bacterial cell lysate. (a) A schematic of OmpG nanopore in absence and presence of lysate. (b) Representative current traces of OmpG^casp pore in the absence and presence of lysates. 160 μL lysate was added to the loop-containing chamber for a final concentration of 15% (v/v). (c and d) Representative single-channel current recording traces and the corresponding all-events histogram of the events generated by (c) casp-3 and (d) casp-7. The postcleavage event is in blue, and the casp-7-OmpG interaction is in red. The average current was calculated from at least four individual pores, and the errors represent the SD. The current percentages of the events were normalized against the current amplitude of the precleaved OmpG^casp pore that was set as 100%. To see this figure in color, go online.