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. 2019 Jul 2;27(9):1597–1611. doi: 10.1016/j.ymthe.2019.06.010

Figure 1.

Figure 1

Genetic Correction of GM-CSF Receptor Signaling in Murine Macrophages

(A) Schematic of the third-generation, self-inactivating (SIN) lentiviral vector expressing the murine Csf2ra cDNA from the short elongation factor 1 promoter (EFS) (mCsf2ra-LV). See Materials and Methods for details. (B) STAT5 phosphorylation assay. Restoration of GM-CSF signaling in mAM cells by lentiviral-vector-mediated expression of both Csf2ra and Csf2rb demonstrated by flow-cytometry-based measurement of GM-CSF-stimulated STAT5 phosphorylation.