Skip to main content
. 2019 Jul 2;27(9):1597–1611. doi: 10.1016/j.ymthe.2019.06.010

Figure 4.

Figure 4

PMT of Csf2ra-LV Transduced BMDMs Restores Alveolar Macrophage Functions

Csf2ra−/− mice received PMT of non-transduced or mCsf2ra-LV-transduced Csf2ra−/− BMDMs and were evaluated at several subsequent times. (A and B) Photomicrographs of primary alveolar macrophages 2 months after PMT of the indicated BMDMs showing macrophage morphology (Diff-Quick stain; phase contrast) (A) and vector-derived GM-CSF receptor α expression (CD116 immunostain, DAPI counterstain; immunofluorescence) (B). Magnification, 20×. Inset, 40×. (C) CD116 expression on alveolar macrophages. Representative histogram of flow cytometry analysis stained with indicated antibody. (D) MFI of CD116 immunostaining of primary alveolar macrophages recovered at the indicated times after PMT. (E–I) Measurement of mRNA transcript levels by real-time qPCR for Csf2ra (E), Car4 (F), Pu.1 (G), Pparg (H), and Abcg1 (I) in primary alveolar macrophages at the indicated times after PMT. In (D)–(I), symbols represent data from one mouse. Error bars indicate mean ± SD for n = 5–8 mice per group (shown) evaluated at each time. *p < 0.05; ***p < 0.001; ****p < 0.001.