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. 2019 Jul 2;27(9):1597–1611. doi: 10.1016/j.ymthe.2019.06.010

Figure 5.

Figure 5

Pulmonary Instillation of Csf2ra-LV Transduced BMDMs without Myeloablation Is Efficacious as Therapy of Hereditary PAP in Csf2ra−/− Mice

Csf2ra−/− mice received one intrapulmonary instillation of mCsf2ra-LV-transduced Csf2ra−/− macrophages and were evaluated after 2, 6, or 12 months in parallel with age-matched, non-transduced WT or Csf2ra−/− mice as indicated. (A) Representative photomicrographs showing lung histology 1 year after PMT and staining with H&E (top), periodic acid-Schiff staining (middle), or Masson’s trichrome staining (bottom). Magnification, 10×, Inset, 40×. (B) BAL fluid turbidity in PMT-treated Csf2ra−/− mice and age-matched, non-treated WT or Csf2ra−/− mice. (C) Total lung surfactant-derived cholesterol recovered BAL. (D–F) Total lung levels of GM-CSF (D), M-CSF (E), and MCP-1 (F) recovered by BAL at 2, 6, and 12 months after one PMT. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.001; ns, not significant.