Table 2.

Effects of Intrapulmonary Instillation of mCsf2ra-LV-Transduced Macrophages on Lung Proinflammatory Cytokine Levels in Csf2ra^−/− Mice

Cytokine in BAL Fluid	Time after PMT (Months)	Untreated Median [IQR] (n)	PMT Median [IQR] (n)	p Value
IL-6, pg/mL	2	0.00 [0.00–16.0] (8)	0.00 [0.00–0.00] (8)	0.4667
	6	0.00 [0.00–0.00] (8)	0.00 [0.00–0.00] (6)	>0.9999
	12	17.8 [6.70–68.5] (13)	5.28 [1.35–19.9] (10)	0.0436
IL-1β, pg/mL	2	0.00 [0.00–8.12] (8)	0.00 [0.00–2.67] (8)	0.8825
	6	0.00 [0.00–0.00] (8)	0.00 [0.00–0.00] (6)	>0.9999
	12	1.05 [0.00–10.7] (13)	7.39 [0.00–22.5] (10)	0.4308
TNF-α, pg/mL	2	3.30 [1.11–5.02] (8)	3.04 [0.04–4.45] (8)	0.5717
	6	0.00 [0.00–0.00] (8)	0.00 [0.00–0.00] (6)	>0.9999
	12	8.26 [5.28–16.3] (13)	4.65 [4.22–7.33] (10)	0.0223

Csf2ra^−/− mice received intrapulmonary instillation of mCsf2ra-LV-transduced macrophages (2 × 10⁶ cells per mouse) (PMT) once and at 2, 6, and 12 months later. BAL fluid was obtained from PMT-treated and age-matched, untreated Csf2ra^−/− mice and evaluated as described in Materials and Methods. Data are presented as medians (interquartile range (IQR), and between-groups comparisons were done using non-parametric methods (Mann-Whitney rank-sum test) for consistency since results for some groups were either not normally distributed or of unequal variance. p values < 0.05 were considered significant.