Fig. 4.

ANN analysis identifies target genes of Wnt signaling during AT2-to-AT1 TD. a Genes identified by ANN analysis depicting the strength of influence. b Gene expression of target genes measured by Affymetrix array. c Gene expression levels of Wnt pathway target genes measured in 3D lung re-aggregate cultures after Wnt ligand treatment (n = 3). Independent t-test, * p < 0.05. d 3D lung aggregate tissues were treated with Wnt4, Wnt5a and Wnt7a ligands then sectioned and stained with anti-ITGAV antibody (pseudo-green), while nuclei were stained DAPI (blue). Size bar is 50 μm. Representative of three independent biological repeats (n = 3). e Densitometry of ITGAV staining (n = 3). Independent t-test, * p < 0.05. f Densitometry of ITGAV staining (n = 3) of the core and the outer layer of the Wnt5a treated 3D lung tissue aggregate cultures (n = 3). Mann-Whitney test * p < 0.05, ** p < 0.01, *** p < 0.001