Figure 3.

Effect of the DNA sequence on the integration reaction by Tn5 transposase. (a) Schematic of the dinucleosome substrates containing the 601 or 603 sequences. (b) The naked DNA (lanes 3, 7, 11, 15) or dinucleosomes (containing 0.01 µg µl⁻¹ DNA, lanes 5,9,13,17) were incubated with the Tn5–DNA complex (0.5 µM). After deproteinization, the DNA fragments were analysed by non-denaturing PAGE with SYBR Gold staining. The bands marked with * are annealing products of the DNA fragments containing partially single-stranded DNA. Black, white and grey arrowheads indicate longer, middle and shorter DNA fragments produced by the Tn5 transposase reaction, respectively.