Figure 1.

Suramin inhibits DNA replication in an in vitro Xenopus replication assay. HTS hits were evaluated for inhibition of [α-³²P]dGTP incorporation into DNA using a cell-free extract prepared from Xenopus eggs at increasing concentrations (10, 50, 150 and 300 µM indicated by black triangle from right to left). No chemical (–) and DMSO were used as negative controls for replication inhibition (far left and far right lanes).