Table 1.
CLas transcription regulators chosen for study
| CLas regulator, GenBank accession no.* | S. meliloti (Sme) ortholog(s)† | Percent identity between CLas and Sme proteins | Regulator type | Putative function of regulator in Sme (ref.) | CLas fold change expression for plant vs. psyllid‡ |
| ACT57084 | RpoH1, RpoH2 | 72, 41 | Sigma factor | Stress response, symbiosis (30–34) | 2.6 |
| ACT57167 | VisN | 50 | LuxR | Motility; forms heterodimer with VisR (35) | 6.7 |
| ACT57166 | VisR | 49 | LuxR | Motility; forms heterodimer with VisN (35) | 4.3 |
| ACT56824 | LdtR (SMc01768) | 70 | MarR | Osmotic stress tolerance, peptidoglycan remodeling (14) | 37.1 |
| ACT56755 | LsrB (SMc01225) | 58 | LysR | LPS biosynthesis, symbiosis (42, 54) | 4.7 |
| ACT56897 | PhrR1 (SMc01110), PhrR2 (SMb21117) | 59, 48 | HTH-XRE | Quorum sensing (43) | Not reported |
| ACT57366 | CtrA | 75 | Response regulator | Cell cycle control; essential gene (40) | 3.3 |
*
Accession numbers are for regulators in the CLas Psy62 genome, assembled from a psyllid metagenome (8). SI Appendix, Table S1 lists other putative CLas transcription regulators not chosen for this study.
†
If the gene name has not been annotated in GenBank, the S. meliloti 1021 unique locus tag is given in parentheses.
‡
Data published in Yan et al. (29). The fold change provided here was calculated from their reported log2 ratio values. Not reported means the gene was listed in Yan et al’s. supplementary table 1 as “selected for qRT-PCR analysis,” but a log2 ratio was not reported.