Fig. 7.

(A) Model fragment showing the connectivity of MRK1 and TIS11 in M1-smart. Nodes are shown with strengths >0.3. MRK1 is involved in modulating the diauxic shift and it mainly interacts with other kinases (FUS3, YAK1, and TPK3) and transcription factors (RDS2, TOS8, and RSF2) rather than enzymes—DAL2 an allantoicase is an exception. HSF1 is its sole parent; it is a trimeric heat shock transcription factor that has previously been implicated in the diauxic shift. (B) Model fragment showing the connectivity of TIS11 in M1-smart. TIS11 is mainly involved in directly controlling metabolic enzymes (CIT2, KGD2, SPS19, CTT1, PFK27, MAE1, TKL2, PFK26), especially those involved in sugar metabolism and the mitochondria. HOG1 is the sole parent of TIS11, it is a mitogen-activated protein kinase involved in osmoregulation. The strongest link is the repression of JEN1, a monocarboxylate/proton symporter of the plasma membrane that has previously been implicated in the diauxic shift.