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. 2019 Aug 19;116(36):18021–18030. doi: 10.1073/pnas.1907447116

Fig. 4.

Fig. 4.

An AD169-specific variant of gB is responsible for caspase-2 activation. (A) Construction of chimeric virus genomes by copy-paste mutagenesis (31). (B) Caspase-2 activation by chimeric virus AD169:AB but not by AD169:ABC. (C) Gene content of fragment C. (D) Sequence alignment of UL55-encoded gB shows an AD169-specific 275Y variant. (E) Amino acid 275 was changed from Y to D in AD169 and from D to Y in TB40/E. Caspase-2 activation by AD169 and TB40/E gB variants was analyzed by immunoblot. The data shown are representative of 3 (B) or 2 (E) independent experiments.