Fig. 6.

Proliferation-defective MavN mutants are impaired for iron uptake. (A) MavN mutant transport activities in vitro correlate with their capacities to support Legionella proliferation as described previously (25). Proteoliposome uptake assays were performed as in Fig. 5. (B) MavN point mutant activities in vitro similarly recapitulate Legionella growth defects depicted in Fig. 3. (C) Transport-defective point mutants mapped onto MavN topology. (D) Effects of Cys ablation on MavN transport activity in vitro. (E) Binding isotherm of MavN C301A titrated with 2.5 mM CoCl₂.