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. 2019 Sep 4;7:e7489. doi: 10.7717/peerj.7489

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© 2019 Fiaz et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Single layered epithelium with columnar digestive cells (DC) from control larvae, with spherical nucleus (N), well-developed brush border (arrows) and basal membrane (arrowheads). Note a regenerative cell (RC) in differentiation. (B) Midgut epithelium of larvae exposed to aqueous solution of LC₅₀ pyriproxyfen showing digestive cells (DC) with cytoplasmic vacuoles (asterisks) and disorganized brush border (arrows). Regenerative cells (RC) from the larvae exposed in concentrations. Note many regenerative cells (RC) in differentiation with large nucleus (black arrow head). L – lumen.