Table 1.

The sequences of used primers

Target	Direction	Sequences (5ʹ to 3ʹ)	Function	Role	Ref.
iroN	F	AAGTCAAAGCAGGGGTTGCCCG	Siderophore receptor	Iron chelator	13
R	GACGCCGACATTAAGACGCAG
fyuA	F	TGATTAACCCCGCGACGGGAA	Ferric yersiniabactin uptake receptor		12
R	TGATTAACCCCGCGACGGGAA
iutA	F	GGCTGGACATCATGGGAACTGG	Ferric aerobactin receptor
R	CGTCGGGAACGGGTAGAATCG
bmaE	F	ATGGCGCTAACTTGCCATGCTG	M blood group antigen-specific M fimbriae	Adhesin
R	AGGGGGACATATAGCCCCCTTC
sfa/focDE	F	CTCCGGAGAACTGGGTGCATCTTAC	S fimbrial adhesins and F1C fimbriae
R	CGGAGGAGTAATTACAAACCTGGCA
sfaS	F	GTGGATACGACGATTACTGTG	S fimbrial adhesins
R	CCGCCAGCATTCCCTGTATTC
focG	F	CAGCACAGGCAGTGGATACGA	F1C fimbriae
R	GAATGTCGCCTGCCCATTGCT
afa/draBC	F	GGCAGAGGGCCGGCAACAGGC	Mannose-resistant hemagglutination
R	CCCGTAACGCGCCAGCATCTC
papG II	F	GGGATGAGCGGGCCTTTGAT	Gal (alpha 1–4) Gal-binding adhesin molecules of P fimbriae
R	CGGGCCCCCAAGTAACTCG
papG III	F	GGCCTGCAATGGATTTACCTGG
R	CCACCAAATGACCATGCCAGAC
fimH	F	TGCAGAACGGATAAGCCGTGG	Type 1 fimbrial adhesin
R	GCAGTCACCTGCCCTCCGGTA
papAH	F	ATGGCAGTGGTGTCTTTTGGTG	P fimbrial
R	CGTCCCACCATACGTGCTCTTC
papC	F	GTGGCAGTATGAGTAATGACCGTTA
R	ATATCCTTTCTGCAGGGATGCAATA
papEF	F	GCAACAGCAACGCTGGTTGCATCAT
R	AGAGAGAGCCACTCTTATACGGACA
kpsMT II	F	GCGCATTTGCTGATACTGTTG	Polysaccharide capsule	Protective
R	CATCCAGACGATAAGCATGAGCA
rfc	F	ATCCATCAGGAGGGGACTGGA	O antigen polymerase
R	AACCATACCAACCAATGCGAG
traT	F	GGTGTGGTGCGATGAGCACAG	Anti-complementary protein of TraT
R	CACGGTTCAGCCATCCCTGAG
usp	F	ACATTCACGGCAAGCCTCAG	Uropathogenic-specific protein (genotoxin)	Invasion	14
R	AGCGAGTTCCTGGTGAAAGC
ibeA	F	AGGCAGGTGTGCGCCGCGTAC	Invasion of the brain endothelium protein A		12
R	TGGTGCTCCGGCAAACCATGC
cnf1	F	AAGATGGAGTTTCCTATGCAGGAG	Cytotoxic necrotizing factor-1
R	CATTCAGAGTCCTGCCCTCATTATT
hlyA	F	AACAAGGATAAGCACTGTTCTGGCT	Hemolysin A
R	ACCATATAAGCGGTCATTCCCGTCA